Biosynthesis of Mycophenolic Acid: Purification and Characterization of S -Adenosyl- l -Methionine:Demethylmycophenolic Acid O -Methyltransferase
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منابع مشابه
Biosynthesis of mycophenolic acid: purification and characterization of S-adenosyl-L-methionine: demethylmycophenolic acid O-methyltransferase.
The final step in the biosynthesis of mycophenolic acid involves the transfer of a methyl group from S-adenosylmethionine to demethylmycophenolic acid. The enzyme, S-adenosylmethionine:demethylmycophenolic acid O-methyltransferase, was isolated from Penicillium stoloniferum and purified 2,700-fold by ammonium sulfate fractionation and diethylaminoethyl-cellulose and Sephadex G-200 chromatograph...
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In angiosperms, OMTs (EC 2.1.1.6) are bispecific and catalyze the methylation of caffeic acid to ferulic acid and 5hydroxy-ferulic acid to sinapic acid using S-adenosyl-L-Met as methyl donor. The resulting products, ferulic acid and sinapic acid, may subsequently be converted to the corresponding alcohols that are incorporated in lignins. In gymnosperms, where syringyl lignins are absent, OMTs ...
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An S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase (SUMT) activity has been identified in Methanobacterium ivanovii and was purified 4,500-fold to homogeneity with a 38% yield. The enzyme had an apparent molecular weight of 58,200 by gel filtration and consisted of two identical subunits of Mr 29,000, as estimated by gel electrophoresis under denaturing conditions. The Km value f...
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Furanocoumarins, Phytoalexins, O-Methyltransferases, Fungal Elicitor, Cell Suspension Cultures Cultured parsley cells (Petroselinum crispum) treated with an elicitor from the soybean patho gen, Phytophthora megasperma f. sp. glycinea, were used as source for the separation, extensive purification and characterization of S-adenosyl-L-methionine: xanthotoxol and S-adenosyl-Lmethionine: bergaptol...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1975
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.8.3.321